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polyclonal antibody against p34 (arpc2)  (Upstate Biotechnology Inc)

 
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    Structured Review

    Upstate Biotechnology Inc polyclonal antibody against p34 (arpc2)
    Arp3 and/or N-WASP knockdowns do not affect cytokinesis . A. HeLa cells were transfected with water (Ø) or specific siRNA directed against N-WASP or Arp3 or N-WASP + Arp3 or ECT2. 72 hours after transfection cells were harvested and lysed. N-WASP, Arp3, ECT2, <t>p34</t> and β-tubulin protein levels were determined by immunoblot analysis using specific antibodies. B. The DNA content of the same HeLa cells was determined by flow cytometry analysis. Arrows indicate abnormal DNA content in ECT2 knockdown cells. A similar scale was used for each histogram. These results are representative of at least three independent experiments. C. Representative DIC images from time-lapse movies of HeLa cells transfected with water (Ø), siRNA directed against N-WASP (N-WASP), against Arp3 (Arp3), against Arp3 and N-WASP (Arp3 + N-WASP) or against ECT2. Images started to be acquired 24 hours post-transfection for 40 hours. Coloured stars indicate the nuclei of dividing cells and of their daughter cells. Time indicated as hours: minutes.
    Polyclonal Antibody Against P34 (Arpc2), supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody against p34 (arpc2)/product/Upstate Biotechnology Inc
    Average 90 stars, based on 1 article reviews
    polyclonal antibody against p34 (arpc2) - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway"

    Article Title: Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway

    Journal: BMC Cell Biology

    doi: 10.1186/1471-2121-9-42

    Arp3 and/or N-WASP knockdowns do not affect cytokinesis . A. HeLa cells were transfected with water (Ø) or specific siRNA directed against N-WASP or Arp3 or N-WASP + Arp3 or ECT2. 72 hours after transfection cells were harvested and lysed. N-WASP, Arp3, ECT2, p34 and β-tubulin protein levels were determined by immunoblot analysis using specific antibodies. B. The DNA content of the same HeLa cells was determined by flow cytometry analysis. Arrows indicate abnormal DNA content in ECT2 knockdown cells. A similar scale was used for each histogram. These results are representative of at least three independent experiments. C. Representative DIC images from time-lapse movies of HeLa cells transfected with water (Ø), siRNA directed against N-WASP (N-WASP), against Arp3 (Arp3), against Arp3 and N-WASP (Arp3 + N-WASP) or against ECT2. Images started to be acquired 24 hours post-transfection for 40 hours. Coloured stars indicate the nuclei of dividing cells and of their daughter cells. Time indicated as hours: minutes.
    Figure Legend Snippet: Arp3 and/or N-WASP knockdowns do not affect cytokinesis . A. HeLa cells were transfected with water (Ø) or specific siRNA directed against N-WASP or Arp3 or N-WASP + Arp3 or ECT2. 72 hours after transfection cells were harvested and lysed. N-WASP, Arp3, ECT2, p34 and β-tubulin protein levels were determined by immunoblot analysis using specific antibodies. B. The DNA content of the same HeLa cells was determined by flow cytometry analysis. Arrows indicate abnormal DNA content in ECT2 knockdown cells. A similar scale was used for each histogram. These results are representative of at least three independent experiments. C. Representative DIC images from time-lapse movies of HeLa cells transfected with water (Ø), siRNA directed against N-WASP (N-WASP), against Arp3 (Arp3), against Arp3 and N-WASP (Arp3 + N-WASP) or against ECT2. Images started to be acquired 24 hours post-transfection for 40 hours. Coloured stars indicate the nuclei of dividing cells and of their daughter cells. Time indicated as hours: minutes.

    Techniques Used: Transfection, Western Blot, Flow Cytometry, Knockdown



    Similar Products

    polyclonal antibody against p34 (arpc2)  (Upstate Biotechnology Inc)
    90
    Upstate Biotechnology Inc polyclonal antibody against p34 (arpc2)
    Arp3 and/or N-WASP knockdowns do not affect cytokinesis . A. HeLa cells were transfected with water (Ø) or specific siRNA directed against N-WASP or Arp3 or N-WASP + Arp3 or ECT2. 72 hours after transfection cells were harvested and lysed. N-WASP, Arp3, ECT2, <t>p34</t> and β-tubulin protein levels were determined by immunoblot analysis using specific antibodies. B. The DNA content of the same HeLa cells was determined by flow cytometry analysis. Arrows indicate abnormal DNA content in ECT2 knockdown cells. A similar scale was used for each histogram. These results are representative of at least three independent experiments. C. Representative DIC images from time-lapse movies of HeLa cells transfected with water (Ø), siRNA directed against N-WASP (N-WASP), against Arp3 (Arp3), against Arp3 and N-WASP (Arp3 + N-WASP) or against ECT2. Images started to be acquired 24 hours post-transfection for 40 hours. Coloured stars indicate the nuclei of dividing cells and of their daughter cells. Time indicated as hours: minutes.
    Polyclonal Antibody Against P34 (Arpc2), supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody against p34 (arpc2)/product/Upstate Biotechnology Inc
    Average 90 stars, based on 1 article reviews
    polyclonal antibody against p34 (arpc2) - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Arp3 and/or N-WASP knockdowns do not affect cytokinesis . A. HeLa cells were transfected with water (Ø) or specific siRNA directed against N-WASP or Arp3 or N-WASP + Arp3 or ECT2. 72 hours after transfection cells were harvested and lysed. N-WASP, Arp3, ECT2, p34 and β-tubulin protein levels were determined by immunoblot analysis using specific antibodies. B. The DNA content of the same HeLa cells was determined by flow cytometry analysis. Arrows indicate abnormal DNA content in ECT2 knockdown cells. A similar scale was used for each histogram. These results are representative of at least three independent experiments. C. Representative DIC images from time-lapse movies of HeLa cells transfected with water (Ø), siRNA directed against N-WASP (N-WASP), against Arp3 (Arp3), against Arp3 and N-WASP (Arp3 + N-WASP) or against ECT2. Images started to be acquired 24 hours post-transfection for 40 hours. Coloured stars indicate the nuclei of dividing cells and of their daughter cells. Time indicated as hours: minutes.

    Journal: BMC Cell Biology

    Article Title: Inhibition of cytokinesis by wiskostatin does not rely on N-WASP/Arp2/3 complex pathway

    doi: 10.1186/1471-2121-9-42

    Figure Lengend Snippet: Arp3 and/or N-WASP knockdowns do not affect cytokinesis . A. HeLa cells were transfected with water (Ø) or specific siRNA directed against N-WASP or Arp3 or N-WASP + Arp3 or ECT2. 72 hours after transfection cells were harvested and lysed. N-WASP, Arp3, ECT2, p34 and β-tubulin protein levels were determined by immunoblot analysis using specific antibodies. B. The DNA content of the same HeLa cells was determined by flow cytometry analysis. Arrows indicate abnormal DNA content in ECT2 knockdown cells. A similar scale was used for each histogram. These results are representative of at least three independent experiments. C. Representative DIC images from time-lapse movies of HeLa cells transfected with water (Ø), siRNA directed against N-WASP (N-WASP), against Arp3 (Arp3), against Arp3 and N-WASP (Arp3 + N-WASP) or against ECT2. Images started to be acquired 24 hours post-transfection for 40 hours. Coloured stars indicate the nuclei of dividing cells and of their daughter cells. Time indicated as hours: minutes.

    Article Snippet: Polyclonal antibody against p34 (ARPC2) was from Upstate Biotechnology.

    Techniques: Transfection, Western Blot, Flow Cytometry, Knockdown